Clone libraries with 35-kb and 3-kb inserts were created by the JGI using their in-house protocols (http://www.jgi.doe.gov/sequencing/protocols), and an additional 3 kb short insert library generated for construction of a shotgun metagenome microarray was constructed by Genome Atlantic.
Tissue microarray was constructed using Formalin-fixed and paraffin-embedded CCA tissues and the matched adjacent normal liver tissues from 42 patients with ALPHELYS MiniCore series 3; 1-mm cores from donor blocks were transferred into a recipient block.
A 70mer oligonucleotide microarray was constructed to analyze genome-wide expression profiles of Corynebacterium jeikeium, a skin bacterium that is predominantly present in the human axilla and involved in axillary odor formation.
METHODS: A cDNA microarray was constructed based on the predicted human retina gene expression profile according to expressed sequence tag (EST) databases.
A custom Affymetrix genotyping microarray was constructed using 2850 SNPs.
The tissue microarray was constructed as described previously [43], [44].
The microarray was constructed from an EST-library of chicken testes and brain, and details of the construction and content of the microarray has been described elsewhere [44].
The DNA microarray was constructed on the S. erythraea strain NRRL2338 genome sequence at whole transcriptome level, and the expression profiles of 6494 ORFs were monitored.
We acknowledge that as a result of the microarray probe design being limited to the six genomes available when the microarray was constructed, our analysis is compromised and will not capture those genes present in many other S. aureus lineages and strains.
The tissue microarray was constructed as previously described [ 9- 11].
A tissue microarray was constructed as previously described [ 14].
