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The strong induction of hsp70 determined by microarray was confirmed by qRT-PCR and by use of transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under control of the hsp70 promoter.
The microarray was confirmed by semi-quantitative real-time PCR (Figure S3).
Up-regulation of this cDNA spot in AE vs. SE with microarray was confirmed with qPCR.
No significant change in Cry1 expression on the microarray was confirmed by real-time PCR.
This significant stability change identified by microarray was confirmed by real-time RT-PCR.
The expression pattern of two genes observed by microarray was confirmed by Northern Blot.
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The expression of OsIPS2 and PHO2/UBC24, which were not supported by the microarray, were confirmed here.
Total RNA was isolated from embryonic day 18.5 and postnatal day 30.5 pairs of eyes from rd7 and B6 mice using TRIzol® and samples testing positive by DNA microarray were confirmed by quantitative RT-PCR.
Expression levels of all these targets identified by the microarray were confirmed by qRT-PCR.
The expression levels of 30 selected genes identified by the microarray were confirmed using real-time PCR.
Significant differences in transcript levels between high and low quality 8CS embryos by microarray were confirmed by qPCR for all 20 differentially expressed transcripts (Table 1).
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