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Microarray was conducted by Nimblegen using a mouse 4-plex expression array (MM8 60mexprxpr ×4).
Recently, a maize custom high-density 105 K Agilent microarray was conducted to investigate the diurnal expression patterns between the leaf and developing ears; about 22.7% (10,037) of expressed transcripts exhibited a diurnal cycling pattern in leaves, but only 0.39% (47) in developing ears [22].
Expression microarray was conducted using samples from 77 ESCC cases (screening group).
The microarray was conducted with the Affymetrix HGU133 Plus2 GeneChip Array.
Labeling of the RNA and hybridization to the above described high-density microarray was conducted as previously described [ 14].
Oligonucleotide microarray was conducted to investigate gene expression profiles in HL and HCC as outlined in supplementary material Fig. S3.
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RNA extraction, labeling, and hybridization for microarray were conducted using infested Kavya with GMB4M and uninfested plants as a control.
cDNA labeling, purification and hybridization against the microarray were conducted as previously described [ 25].
Probe synthesis and hybridization to microarray were conducted twice per independent experiment resulting in 12 independent Cy5/Cy3 intensity ratio data points for each spotted cDNA at each time point.
Hybridization to the microarrays was conducted for 16 h at 45°C.
Fabrication of microarrays was conducted concurrently with EST sequencing, and we have sequenced 1,546 high-quality clones on the array to date.
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