RNA for microarrays was collected using RNeasy Mini kits (Qiagen) and amplified and biotin-labeled using the MessageAmp-II Bacteria RNA amplification kit (Ambion) per the manufacturer's instructions; 10 15 ng of template RNA was used per reaction.
Under centrifugation, the synthesis and the 2D VO2 microarrays were collected from the aqueous phase.
High throughput gene expression data from spotted cDNA microarrays are collected by scanning the signal intensities of the corresponding spots by dedicated fluorescence scanners.
All mosquitoes used for the genotyping and microarrays were collected in June of 2011 and July of 2012 from breeding sites as larvae and reared to adults at the insectaries of CNRFP.
Microarray data was collected by confocal scanning using the Roche NimbleGen MS200 Microarray Scanner at 2 μm pixel resolution (Roche NimbleGen, Inc., Madison, WI, USA).
It implies that these progression-related sub-networks, especially the ones which are annotated with significant functions, can be helpful in the understanding of molecular mechanism that underlies the progression of HCV induced hepatocarcinogenesis. Microarray data was collected from Gene Expression Omnibus (GEO) database.
Briefly, raw microarray data was collected from NCBI's Gene Expression Omnibus (GEO) [25], [26], and features on the array were mapped to NCBI Entrez Gene IDs using Version 9 of the mapping provided by Dai et al. [27], yielding 16,297 gene probe sets and 64 Affymetrix control gene probe sets.
Microarray data was collected by Roche NimbleGen (Iceland) as a service using 4 × 72 k format arrays.
Microarray data was collected at Asuragen using GeneChip Affymetrix HuGene10stv1_Hs_ENTREZG_desc array, according to standardized operating procedures.
Blood used for RNA microarray analysis was collected from the present cohort via venipuncture into PAXgene tubes (QIAGEN Inc., Valencia, CA).
