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Exact(3)
For each of the existing GO terms, the cumulative number of genes meeting our criteria (e.g. up- or downregulated) of all genes represented in the microarray was calculated.
Fold change for the expression value for each gene and microarray was calculated relative to the arithmetic mean for the vehicle group for that gene.
The frequency of genes of a given category on the microarray was calculated as the ratio of the number of genes of this category divided by the total number of genes on the microarray, and the frequency of regulated genes of a given category was calculated as the ratio of the number of regulated genes of this category divided by the total number of regulated genes.
Similar(57)
The sensitivity and specificity of our microarray are calculated by following formulas: (a) (b).
The log2 ratios of each spot in the microarray were calculated from the raw signals obtained from both Cy5 and Cy3 channels, and then the values were scaled by Tukey bi-weight mean.
The mean of the total gene signal for all the miRNAs on the microarray were calculated for each individual hybridization, and the mean and standard deviation of these for each prep type in each cell line are shown.
For every microarray, duplicate spots were averaged, and then the average expression of every gene across all technical replicate microarrays was calculated.
Spearman correlation between R and G channels from replicate microarrays was calculated in order to select unbiased microarray data for significance analysis.
Expression levels obtained by microarrays were calculated as the log ratio sample vs universal reference RNA.
Global Pearson correlation coefficients for microarrays were calculated using the statistical software package R limma[ 27].
The resulting expression levels for each of the miRNAs measured by the microarrays were calculated and filtered for miRNAs expressed above a background level (background was set at 30, approximating the median signal per array).
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