The microarrays were assembled with hybridization caps and rehydrated with RNase-free water at 65C for 10 minutes.
Microarrays were assembled using clones obtained during the EC "Zeastar" project (http://www.cerealsdb.uk.net/zeastar.htm).htm
Tissue microarrays were assembled and subjected to immunohistochemical analysis with a panel of 18 antibodies.
Tissue microarrays were assembled as described previously (Bubendorf et al, 2001) with three replicate cores for each tumour and one core for each normal mucosal sample.
Tissue microarrays were assembled as in [ 23] for immunological analysis of β-catenin (clone17c2 Novocastra Laboratories Ltd. Newcastle upon Tyne, UK), M30 (M30 CytoDEATH Roche Diagnostics GmbH Mannheim Germany) for apoptosis and KI67 (clone M1B1, Dako, Glostrup, Denmmark) for proliferation.
In this illustration, microarray data was assembled over a span of 5 days from the lungs of mice infected with influenza virus on day 0 (lung tissues are illustrated in Figure 8A).
The microarray slide was assembled in a Gentel SIM plex 16 Multi-Array System device (Gentel biosciences, cat# 4 1007) with custom modifications including a modified bottom gasket and top spacers (Additional file 1: Figure S1A).
qPCR analysis of SNRK expression in hESC CM samples as well as all Microarray validations was assembled in TaqMan gene expression master mix (Life Technologies) and run in a Bio-Rad real-time thermal cycler according to manufacturer's instructions.
A tissue microarray (TMA) was assembled using 100 HCC cases, consisting of 50 cases each of HCC with or without metastasis studied over a follow-up period of 2~5 years.
Tissue microarrays (TMAs) was assembled by taking three core needle 'biopsies' from defined tumor regions in the preexisting paraffin-embedded tissue blocks, using techniques and an apparatus developed by Beecher Instruments Inc., Micro-Array Technology (Sun Prairie, WI, USA).
