Sentence examples for microarray validation by from inspiring English sources

For microarray validation by WISH, an etsrp-mcherry construct was generated by sub-cloning mcherry from mcherry-tol2 (a gift from Hong Zhang) into pSPORT6 between XbaI and XhoI, and etsrp was subsequently subcloned into this vector at the 5'end of mcherry between BamHI and XbaI sites.

SB performed microarray validation by qPCR.

For the microarray validation by qPCR five different animals of each group were used.

Here, we report our experience with microarray validation by qPCR on AA-aRNA and we present an optimized protocol that improves the reliability of this validation.

The microarray validation by independent quantitative real-time PCR (qRT-PCR) on some of the genes was performed (data not shown) as described in our earlier publications [ 68, 70].

SPK drafted the manuscript and carried out the cell cultures, sampling, radioimmunoassays, microarray hybridizations, microarray validations by real time PCR, statistics, and all data analyses.

Genes used for microarray validation.

We randomly selected 15 DEGs identified by microarray for validation by quantitative Real Time PCR (qRT-PCR).

We randomly selected 18 differentially expressed genes with at least 2-fold change identified by microarray for validation by quantitative Real Time PCR (qRT-PCR).

A set of eight genes was chosen for microarray data validation by real time quantitative reverse transcription PCR (qRT-PCR).

Microarrays and validation by quantitative real-time polymerase chain reaction (qRT-PCR).