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A microarray using the Illumina Direct Hybridization Assay (performed by ServiceXs, Leiden, The Netherlands) was performed on purified whole blood RNA samples from two patients with classical SchS and 1 IgG variant case with myeloid-restricted NLRP3 mosaicism.
DNA methylation was also tested on this microarray using the HpaII tiny fragment enrichment by ligation-mediated PCR HELPP) assay.
Detection of the four herpesviruses was set up in a microarray using the recombinant proteins of herpes simplex virus (HSV) glycoprotein G1 and G2, varicella-zoster virus (VZV) glycoprotein E, and cytomegalovirus (CMV) pp150 phosphoprotein.
We report the progress on 765 bp full green fluorescent protein (GFP) gene assembly of oligonucleotides from a single DNA microarray, using the amplification and assembly of chip-eluted DNA (AACED) strategy.
For that purpose, we treated differentiated Caco-2 cells with micelles containing the assayed lipids (cholesterol, conjugated linoleic acid and docosahexaenoic acid) and the screening of miRNAs was carried out by microarray using the μParaflo®Microfluidic Biochip Technology of LC Sciences (Huston, TX, USA).
Using a standard 96-well plate, the user can load any combination of reagents and direct their introduction onto the microarray using the scripting program.
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Classification of the Rembrandt samples was then performed using prediction analysis for microarrays using the signature gene class centroids [11].
RNA was then converted to labeled cDNA, and hybridized to microarrays using the 5-hour time point as a reference sample for the other two time points.
Lastly, we performed the analysis of time-course microarrays using the EDGE [16] and deleted those that did not change with time.
Firstly, quality control analysis was performed on all microarrays using the Simpleaffy software package [ 79].
The newly designed microarrays used in this study were custom-made pan- Bordetella microarrays using the 8 × 15 K format developed by Agilent Technologies, Wilmington, USA.
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