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For example, in a two-color microarray, transcripts extracted from different conditions are labeled with distinct fluorescent dyes while being converted to cDNA.
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Three homemade cDNA microarrays have been designed using transcripts extracted from equine joint cartilage and synoviocytes [ 27- 29].
The microarray was used to measure abundances of transcripts extracted from wild-type and a Crp mutant (52).
As with the proteins and microarray transcripts, many of the top transcripts were the same PR proteins in the microarrays.
Microarrays were then imaged on an Agilent microarray scanner and extracted using Agilent Feature Extraction 9.5.
Microarray data were extracted from scanned GeneChip images and analyzed using Microarray Suite version 5.0.1 (Affymetrix).
Microarray data were extracted and analyzed as previously described [27].
Bound RNA transcripts were extracted with DynaMagTM-Spin (Invitrogen).
For each microarray hit, we extracted and classified COUP-TF HMM matches within any of 3 proximal regulatory regions: 1) promoter; defined here as 5000bp upstream of each transcription start site associated with the gene, 2) 5' UTR, and 3) first intron of all transcripts associated with the gene.
The mRNA transcripts were extracted from ENSEMBL.
anilis transcripts were extracted from these results.
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