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Microarray transcriptional analysis was performed in triplicate using the HumanWG-6 v3.0 Expression BeadChip system (Illumina, San Diego, CA).
In this regard, using microarray transcriptional analysis we have previously characterized time-dependent changes in the gene expression profile of cultured human myotubes mostly affecting contractile and apoptosis-related genes [40].
For microarray analysis, RNAs from triplicate independent cultures of vector-infected HPRT knockdown and control fibroblasts were prepared separately, pooled and used to prepare cRNA and finally subjected to microarray transcriptional analysis in triplicate.
Among the streptococci, competence development is best characterized in S. pneumoniae where 23 genes have been determined by insertional mutagenesis and whole-genome microarray transcriptional analysis to be individually essential for transformation [69].
We have used microarray transcriptional analysis to examine the acute effects of IGF-1 on global patterns of gene expression in C2C12 myoblasts and have identified approximately 157 genes that are up-regulated and 75 genes down-regulated from 2- to 6-fold after treatment with IGF-1.
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Linear amplification of RNA was used to obtain enough material to perform microarray based transcriptional analysis of the pre-erythrocytic stages.
While microarray-based transcriptional analysis is a useful tool for functional genomics in fish, to date there has been no microarray platform available for catfish species.
Microarray-based transcriptional analysis revealed up-regulation of self-renewal and pluripotency-associated genes [38] [40] in AFiPSCs in contrast to primary AFCs (Figure 2A).
We used a Gene-ontology-driven microarray-based transcriptional analysis coupled with protein-level and activity assays to identify genes and pathways involved in Mk apoptosis.
Microarray-based transcriptional analysis was carried out for samples at each timepoint, using the 'reference' design [ 53], with Human Thymus Total RNA (Ambion, Austin, TX) as the reference RNA.
DNA-microarray-based transcriptional analysis was carried out using the mixed-cell population in Mk cytokine cocktail cultures from days 0 – 4 and, starting on day 5, using CD41a+ cells enriched to above 97% by positive-selection using immunomagnetic beads.
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