The microarray transcription profiles were confirmed by Northern blot analysis using 2 genes in Group 1 (Contig127, Contig12) and 5 genes in Group 2 (Contig488a Contig34new Contig533ew, Contig508, Contig508) (See Additional file 5).
A limited amount of total RNA was available and we used a T7 RNA polymerase based linear amplification method that has been shown to have no systematic influence on microarray transcription profiles [ 67].
We applied DNA microarray transcription profiling to 48 individual F1 trees from the O3 × R5 cross and identified transcripts with expression levels associated with PM disease and WAA pest resistance phenotypes.
Microarray transcription profiling analysis of larval midgut tissue, where Lerp is very highly expressed normally (Brown et al., 2014; Dos Santos et al., 2015), indicates that Lerp transcripts containing the 3′ exon are detectable in mutant midgut cells at ca. 27% of wild type levels (M.H. and J.C.E., unpublished data).
A comparison of the microarray based transcription profile of cells transfected with vector and 59Q showed distinct differences.
In an early oligonucleotide microarray-based transcription profile analysis including 50 hepatocellular nodular lesions ranging from low-grade dysplastic nodules to primary HCC, Cbx4 was shown to be among the top clusters of genes that were highly correlated with ostensible biological implications.
Our approach allows us to incorporate a wide variety of input data, including homology information, genome context, predicted transcription factor binding sites, and microarray transcript profiles.
Using microarray technology, transcription profiling was performed for putative lung CSCs isolated from the two lung cancer cell lines.
Current approaches in functional genomics based on protein- protein interaction and microarray based transcription profiling are helping to decode this mystery.
Microarray based transcription profiling for understanding the pathophysiology of sepsis has predominantly focused on the blood profiles from sepsis patients or animal models [ 2- 4], with few reports on the primary organs in which the infection originated, such as lung [ 5] and brain [ 4].
