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Different stress conditions, tissues and plant species, from Arabidopsis to cereal crops [ 10- 17], have been investigated with microarray tools to find drought-regulated genes.
With the goal of finding molecular signatures that can be used for early risk assessment, these projects employ microarray tools to assay for gene expression responses to cancer-related chemicals.
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Furthermore, different and improved microarray platforms have been used over time, due to the increased availability of improved microarray tools tailored to the genome sequence of most livestock species.
More generally, we find that RNA-Seq can be used as a tool to evaluate microarray probe quality, filter out bad probes, and improve the utility of microarrays as tools to measure absolute gene expression levels.
Microarray analysis using tools to recognize patterns of response have been utilized in the cancer field to develop biomarker panels of transcripts for diagnosis and selection of treatments most likely to be effective.
Therefore, in the future, we will use the cDNA microarray and other tools to examine the difference between the control cell and the K -ras transfected cell to understand the cross talks between the Ras-to-MAPK pathway and the regulation of the steroidogenesis process.
As the ultra high throughput sequencing technologies become more affordable and acceptable, more and more organisms will be sequenced at the transcriptomic level, leading to an increasing demand for making microarray tools in the foreseeable future.
To support experimental transcriptome profiling, macro- and microarray tools have been constructed for the two model mycorrhizae, based either on PCR-amplified cDNAs or 70mer oligonucleotides.
Microarrays, as efficient tools to simultaneously monitor the expression of tens of thousands of genes, have been widely applied in both mechanistic and decision-making research during the past decade [1] [4].
Motivation: High-density DNA microarrays provide useful tools to analyze gene expression comprehensively.
DNA microarrays are excellent tools to elucidate the role of these genes in plant defense [ 12, 13].
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