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Illumina's BeadStudio version 3.1 was used to generate signal intensity values from the scans, subtract background, and scale each microarray to the median average intensity for all samples (per-chip normalization).
After converting the probe list from the 244K feature Agilent microarray to the latest genome build (NCBI 36.2), 15,684 regions were identified which, for at least one individual, lay between a probe previously scored as copy number variable and a probe which was unchanged compared to the reference sample i.e.e
Applying significance analysis of microarray to the combined set of our 5 pairs of ULM and matched myometria and the GSE593 data, we identified 2674 probe sets (1,117 up- and 1,557 down-modulated) that were significantly different between ULMs and matched myometrium controls (Figure S1).
Comparison of the 027-specific probes on the microarray to the published sequence of R20291 has revealed some differences.
We mapped the 41,174 non-control probe sequences from the Whole Mouse Genome Microarray to the mouse reference genome (NCBI37, downloaded March 2011) using BLAT ((Kent, 2002); minScore = 55, default settings for all other options).
For the real time RT-PCR analysis, a lean control group was included to compare C5L2 gene expression (a gene not on the microarray) to the morbidly obese subjects.
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Here we describe the application of spotted cell microarrays to the study of human cell lines.
Radioresistant sublines were established by FIR, and we applied cDNA microarrays to the parent and radioresistant sublines.
Is it therefore legitimate and possible to use the red blood cell's microarray in addition to the platelet's microarray to infer the platelet's network?
These samples were first used for the miRNA microarray to obtain the miRNA profiles.
We used a custom-made focused cDNA microarray to determine the adipose tissue mRNA expression profile.
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