This disagreement can be accounted for by the limited ability of cDNA microarrays to discriminate between transcripts of structurally similar members of multi-gene families.
Although we did observe some cases of individual FLcDNAs mapping to multiple array elements, as well as multiple FLcDNAs mapping to the same array element, it should be noted that the in silico match stringency applied here is likely higher than the capability of cDNA microarrays to discriminate among highly similar transcripts by actual DNA hybridization.
Recognizing the power of microarray approaches to discriminate breast cancer patients into clinically informative groups, several studies aimed at using clustering approaches to tackle the prognostication problem (Table 1).
Even though a NimbleGen microarray is used to discriminate homeologous genes in polyploid species [ 53], the microarray chip used during this study was not designed to distinguish wheat homeologous genes.
In addition, the microarrays were able to discriminate between closely related viral strains in a number of cases.
Others (e.g. PFGE, MLST, AFLP, VNTR, fla sequencing, DNA microarrays) are able to discriminate between strains [ 8, 14, 29, 32- 34].
No removal of eukaryotic RNA was performed as the specificity of the microarray was high enough to discriminate between different S. aureus strains.
The combined microarray offers an opportunity to discriminate between individual trees as well as analyze transcript abundance, variability and the usefulness of the chip for fingerprinting.
A DNA microarray was designed based on this sequence, and initial tests revealed that two prophages and a plasmid present in the genome of strain ATCC 3502 were absent from 11 test strains of proteolytic C. botulinum and C. sporogenes, and that the DNA microarray could be used to discriminate between strains of proteolytic C. botulinum [ 14].
Microarray technology has been used to discriminate differences in gene expression profiles in tissues and PBMCs.
