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In this study we have adapted a statistical method that utilizes absolute signal intensities from a reference set of positive controls and negative non-homologous control sequences to determine the absolute intensity range in each channel that may be used with a certain confidence level on a particular microarray to calculate expression ratios.
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This used the log2 intensity ratio microarray data to calculate the median log2 intensity ratios for each C. sakazakii BAA-894 gene.
This particular tool uses hybridization signal intensities from microarray experiments to calculate a Pearson correlation coefficient (r-value), which is a scale-invariant measure of expression similarity.
We then used the TRAP microarray data to calculate the IP/UB ratio for genes previously identified as enriched in astrocytes, neurons, mature oligodendrocytes (MOs), oligodendrocyte precursor cells (OPCs), microglia, endothelial cells or pericytes [ 25].
The TransCount method developed by Frigessi et al. [ 10] is based on Bayesian statistical modeling and utilizes covariates of the microarray experiment to calculate the concentration from the signal intensity of each probe on spotted arrays.
The Affymetrix Microarray Suite version 5.0 program was used to normalize microarray data and to calculate signal intensity, detection p-value, signal log ratio and change p-value using the statistical algorithms of version 5.0.
The next step involved microarray significance analysis to calculate significant differences in the transcript levels.
These ratios were then processed as were processed the microarray intensity ratios to calculate Trep values and generate a replication timing profile.
It is during this calculation that the fluorescent signal intensities at each location on the microarray are indexed to calculate relative allele signal (RAS) values.
The individual's DNA was also genotyped on a microarray in order to calculate the allelic frequencies of the spiked pools.
Linear regression analyses were used to assess differences in gene expression in microarray experiments and to calculate the percentage of R- to S-flurbiprofen inversion in experiments using continuous oral treatment.
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