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These results showed the microarray to be a suitable tool for studying methanogenic communities in sludge.
MAP3K7 was predicted by the microarray to be repressed to a greater extent by pulsatile cortisol, and this effect was not confirmed by qRT-PCR measurement.
Primers were designed for twelve genes that were found by microarray to be differentially expressed between the two conditions at 72 hours; seven genes were upregulated and five were downregulated in the forskolin condition.
MAOA was predicted by the microarray to be induced to a greater extent by continuous cortisol, and this was seen by qRT-PCR, though it did not reach statistical significance, and FOXO1 was induced to a similar extent by both pulsatile, and continuous cortisol in both the array, and the qRT-PCR.
This enables the microarray to be used with different labeling protocols.
Each participant received six Rat230 2.0 GeneChips (Affymetrix, Inc, Santa Clara California, USA) for use in each study, one microarray to be used for each RNA sample.
Similar(41)
The expression of miR-133a and miR-133b was first observed in microarrays to be significantly upregulated during myoblast differentiation.
However, expression levels of key responding genes of the microarray need to be validated by real-time PCR.
Protein microarray is considered to be one of the key analytical tools for high-throughput protein function analysis.
In our experiments, the microarray was found to be more sensitive than the qRT-PCR data.
Twenty probes in the microarray were found to be modified by one of the factors.
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