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The Verigene 2C19/CBS Nucleic Acid Test is a fully automated microarray test that utilizes gold nanoparticle-conjugated oligonucleotide probes to detect nucleic acids captured by array probes, and this method eliminates the need for target amplification, namely, a PCR step, before array hybridization [ 3, 15].
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We developed a microarray-based test that uses a FMD DNA chip containing 155 oligonucleotide probes, 35 45 base pair (bp) long, virus-common and serotype-specific, designed from the VP3-VP1-2A region of the genome.
We chose 14 SYNZIPs for in vitro testing, selecting proteins that had many interaction partners in the prior coiled-coil microarray tests or that interacted with a SYNZIP that had many interaction partners.
When he was 3, they tried microarray analysis, a genetic test that is nowhere near as sensitive as sequencing.
TargetPrint™ (Agendia, Irvine, CA, USA/Amsterdam, The Netherlands) is a microarray-based gene expression test that allows quantitative assessment of ER, PR and HER2 at the RNA level in breast cancer.
The aim of the study is to confirm that the microarray test will save up to 30% of the patients from unnecessary chemotherapy and to identify 5% of them who are nowadays 'undertreated'undertreated
(SBC), which performed the microarray test.
A new generation of statistical tests has been developed for the microarray context in recent years, introducing tests that borrow information between genes using empirical Bayes and other statistical means (Baldi and Long, 2001; Efron et al., 2001; Lönnstedt and Speed, 2002; Smyth, 2004; Tusher et al., 2001; Wright and Simon, 2003).
There are over 2500 genes known in the donor region for the congenic strain, so as in previous examples, after QTL mapping, microarray testing was the single step that reduced the number of candidate genes the most.
In a retrospective screen of 9773 individuals referred for microarray testing, Rosenfeld et al found that 77 carried chromosome 16p11.2 anomalies (45 deletions, 32 duplications, 0.78%).
Supplementary in vivo experiments involving for example studies of expression patterns by Northern blotting or microarray testing are still essential to confirm that the sRNA candidate genes are indeed transcribed; such studies also provide information about temporal expression patterns, potential precursor forms and degradation products.
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