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PD, JF and JG carried out the microarray test and the bioinformatics analysis.
The combined use of the developed microarray test and the TB-Biochip (MDR) allows the simultaneous identification of the MDR and XDR causative agents and the detection of their resistance profiles in a single day.
The combined use of the developed microarray test and the previously described microarray-based test for the detection of rifampin and isoniazid resistance allows the simultaneous identification of the causative agents of MDR and XDR and the detection of their resistance profiles in a single day.
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VD performed the microarray tests and analyzed the data.
FAC carried out the microarray testing and performed the statistical analysis.
At enrollment, additional samples were collected for microarray testing and immunological assessments (see below).
Molecular subtypes can be defined by microarray testing and this classification approximated using IHC results of ER, PgR, HER2 and other proliferation biomarkers.
However, DNA microarray testing and analysis has evolved over many years through studies such as the MicroArray Quality Control (MAQC) project [ 65, 66] and further studies and cost reductions in sequencing are similarly required to develop RNA-seq analyses.
The QRT-PCR testing phases are designed to use the same pools of transcripts developed for microarray testing and to evaluate the candidate external RNA controls on multiple QRT-PCR platforms.
As demonstrated in Fig 2, our DNA microarray test systems and the experimental cell culture replicate samples were highly reproducible and consistent.
In all validation steps, only 5 genes amplified by qPCR were analyzed in the microarray test datasets and no further gene selection was used in the cross-validation loops.
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