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These other studies, however, were done using crude tissue blots of undefined self-molecules; the defined-antigen microarray technology used here apparently made it possible for us to detect the changes in fine specificity of the autoimmune repertoire occurring subsequent to birth.
With the microarray technology used (a 16k library for coverage of 20 25 k genes), it is obvious that not all gene products affected by the MO treatment are included in the presented data set.
Another limitation is related to the tissue microarray technology used in this study might also contribute to some limitations of the work.
It also demonstrated the reliability of the microarray technology used in the current study and also provided a greater confidence in our research results.
The program uses well-established thermodynamic models of hybridization in solution, as the more complex surface effects are still under active study and are also dependant on the microarray technology used.
For example, the scoring is independent of the microarray technology used (although we have used data only from Affymetrix chips), and thus data obtained through different technologies could be compared in the same analysis.
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Because of the relatively small sample size and relatively poor signal/noise ratio for genes with low expression levels, most microarray technology using human blood or monocyte samples has produced results that have not been replicated well.
With the help of microarray technology using P. falciparum transcription, it was demonstrated that all glycolytic enzymes are upregulated at the early trophozoite stage during the asexual cycle, coinciding with the time of maximal metabolic activity by the parasite [ 15].
Headquartered in San Diego, California, Illumina develops microarray technologies used in large-scale analysis of genetic variation and function.
Recently, a comparison was made of two microarray technologies used in the detection of CNVs.
The Affymetrix GeneChip® gene expression analysis array, one of most commonly used microarray technologies, uses multiple oligonuleotides (25-mers) to measure expression abundance of a single gene.
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