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With the continuing advancement of protein microarray technology, this microarray could be expanded to hundreds or even thousands of allergens, providing an efficient and economically feasible assay to measure allergen-specific IgE in sera.
Transcript levels for CCR7 were the lowest ones among those for the 10 selected genes by using RT-qPCR, and very low signal intensities were measured for CCR7 by using the microarray technology; this indicates that differences in gene expression are more difficult to quantify by using microarray technology for low transcript levels.
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Microarray technology accomplishes this purpose.
On the basis of using the rat adjuvant-induced arthritis model of human RA and microarray technology in this study, we describe the gene expression profiles of arthritic LEW rats at different phases of the disease as well as the modulation of gene expression by a tolerogenic disease-protective regimen employing the disease-related antigen Bhsp65.
Instead, this study addresses the validity of using microarray technology for building this foundation by applying consistent analysis to evaluate reproducibility.
Asmann et al. (2009) comparing DGE-tag with the microarray technology, emphasized that this method has a high sensitivity and reproducibility for transcriptome profiling [23].
We used cDNA microarray technology to achieve this aim, representing the first time such an approach has been applied to any euphausiid species.
Another limitation is related to the tissue microarray technology used in this study might also contribute to some limitations of the work.
Microarray technology presented in this paper yielded an in depth analysis of diversity for lettuce germplasm separating even closely related lines such as the crisphead class.
As genes expressed at near-background levels have historically been problematic for microarray technology [ 22], this aspect of the CAD transcriptome may benefit greatly from the increased dynamic range of RNA-Seq.
With this microarray technology, studying thousands of genes simultaneously becomes possible.
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