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Currently, many gene-expression studies use microarray technology, the basis for which is RNA transcript hybridizing to immobilized probes which correspond to tens of thousands of defined sequences.
More recently Carmichael and colleagues studied by microarray technology the genomic patterns in acute ICH (<24 hours) compared to healthy brains.
Compared to microarray technology, the RNAseq method offers several distinct advantages.
Compared with the previous microarray technology, the RNA-seq approach offers several obvious advantages.
Furthermore, with the continuing development of microarray technology, the capacity of microarrays is expected to increase.
The ChIP-on-chip technique combines chromatin immunoprecipitation (the ChIP) with DNA microarray technology (the chip).
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For these microarray technologies, the number of available tools is small compared with mRNA analysis tools.
For most microarray technologies, the set of potential targets must be available in advance so that appropriate probe sequences can be used.
The main drawback of the microarray technology is the necessary large initial investment for sophisticated and expensive instruments needed for microarray printing and laser scanning.
Here we provide a review of the microarray technology, including the introduction of array platforms, experimental designs, RNA isolation and amplification, array hybridization, and data analysis.
We then applied a loess normalization procedure within arrays to remove any systematic trends which arise from the microarray technology from the methylation measures [24].
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