Common techniques including microarray approach and analysis of transcriptome databases are the major ways to globally identify cis-NATs in various eukaryotic organisms.
RNA analysis has generally been performed on snap-frozen or fresh materials, using variable techniques including microarray, northern blotting and RT-PCR.
Since their discovery, miRNA analysis has generally been performed on snap-frozen or fresh samples, using variable techniques including microarray, northern blot analysis and PCR [ 2].
Protein or mRNA expression profiling has been shown to permit the molecular classification of breast cancers via a range of techniques including cDNA microarray, quantitative RT-PCR and tissue microarray (TMA) into consistently observable groupings [ 7- 10, 12- 19].
We intend to use more sophisticated techniques including microarrays in a group of patients undergoing treatment in a randomised setting, with the ultimate hope of being able to further sub-classify bladder tumours into clinically homogenous groups.
At present, research has focused on the molecular interaction between plant and pathogen using various techniques, including microarrays [ 13], representational difference analysis (RDA) [ 14], suppression subtractive hybridization (SSH) [ 15], cDNA-amplified fragment length polymorphism (cDNA-AFLP) [ 16], and serial analysis of gene expression (SAGE) [ 17].
As a model eukaryote with a compact genome, the budding yeast was integral in the pioneering of many high-throughput techniques, including DNA microarrays (8), chromatin immunoprecipitation coupled with microarrays (ChIP-chip) (9), genome-scale nucleosome position mapping (10), high-resolution transcriptome mapping (RNA-seq) (11) and continues to be an important testing ground for new innovations.
The last decade has witnessed the emergence of cheap, high-throughput techniques, including the microarray expression platforms.
The core laboratory served as a training ground for faculty and students to learn new techniques including phenotype microarray analysis, DNA sequencing, and RT-PCR.
In the current study we applied various techniques, including Affymetrix microarray hybridization and semiquantitative RT-PCR, to identify genes expressed differentially in renal oncocytomas.
