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Expression analysis done by tissue microarray technique shows that if the expression of HBsAg, HBcAg, p21 and Rb proteins in HCCs is downregulated, the possibilities of HCC development will increases as these proteins are also tumor suppressors [ 10].
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Further confirmation of that observation comes from recent studies made with the use of the cDNA microarray technique, showing that breast cancers may vary considerably in their molecular profile [ 24, 25].
Microarray techniques show promise in this regard, as their high throughput nature can potentially detect a range of viruses using a single test.
Using the microarray technique, we have shown that Sgk1 mRNA expression is consistently altered in the medial prefrontal cortex of chronically stressed mice.
A recent broad study using tissue microarray technique questioned these results by showing similar ILK expression in tumor tissue and normal tissue [36].
In these cases positive psoriasin and calgranulin immunostains correlated with their gene upregulation as shown using DNA microarray technique (Seth et al, 2003).
The pattern and magnitude of expression for each validation gene (LGALS3, galectin 3; microfibrillarbrillassociatedted protein 5; protein protein kinase, DNA-activated, catalytic polypeptide 1) compared to house keeping controls was identical using PCR and microarray techniques (data not shown).
Because of microarray technique or other unknown factors, some probes show higher expression level at 3h than at 0h.
These studies have shown the importance of high-throughput transcript profiling, such as the DNA microarray technique (DeRisi et al., 1997), for gaining insight into the ischemic brain.
Microarray technique is a useful tool to identify functional gene candidates.
We used microarray technique for this purpose.
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