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Microarray technique is a useful tool to identify functional gene candidates.
One limitation of the standard microarray technique is that it can only detect the relative cDNA expression levels of two samples.
The microarray technique is usually used by researchers to identify differentially expressed genes between two different RNA samples.
This suggests that the microarray technique is able to provide additional information in fetuses with CNS malformations and a normal karyotype.
This difference likely resides in the higher number of sampling times considered in the cDNA-AFLP study and in the fact that the microarray technique is less sensitive than the PCR-based cDNA-AFLP technique.
The most noticeable weakness of microarray technique is that it still suffers from poor detectability and reproducibility for low-copy and transiently-expressed genes [ 38]; the latter are actually very important as they are most likely enzymes and transcription factors, performing transient yet critical biological functions.
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cDNA microarray technique was used to evaluate the effects of these synthetic compounds on 112 genes involved in apoptosis pathways.
An electronic deoxyribonucleic acid (DNA) microarray technique was developed for detection and identification of viable Escherichia coli O157 H7, Vibrio cholerae O1, and Salmonella typhi.
Microarray technique was employed to analyze affected genes and revealed that 481 genes were expressed at higher levels in the mutant strain and 188 genes were expressed at higher levels in the control strain under normal growth conditions, indicating that transcriptomic changes of rice seeds are induced by the space environment.
The potential of microarray technique was used to analyze the global response of P. tricornutum fusiform cells to the availability of silicic acid.
This differential expression of BMP-4 and BMP-5 as determined by microarray technique was verified by semiquantitative PCR.
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