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Most studies used microarray technique for genome wide profiling, but other methods to detect genomic changes and microRNA are gaining interest.
In this present study, a new method based on the allele-specific extension on microarray technique for detecting changes of DNA methylation in cancer was developed.
We used microarray technique for this purpose.
Intratumor heterogeneity also limits the informative value of the widely used tissue microarray technique for studies on novel prognostic or predictive biomarkers.
Here, we describe the optimization of a miniaturized cell spot microarray (CSMA) method, which facilitates utilization of the transfection microarray technique for disparate RNAi analyses.
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In particular, topics regarding (1) the soft-lithography technique to prepare micro-patterned substrates for the spatial control of cell adhesion, (2) biomaterials stiffness-dependent cellular responses, and (3) the microarray techniques for analysis of cell/biomaterials interactions are discussed.
Real-time RT-PCR is an alternative to microarray techniques for the analysis of transcription from multiple genes.
There have been many reports using microarray techniques for rapid and high throughput validation of the subtraction specificity of SSH by spotting the enriched clones on the chips or membranes [ 10- 15, 15- 23].
In the work reported here, we performed a comprehensive set of experiments to measure the regulation of endogenous PR target genes using current microarray techniques for whole genome expression profiling in T47D cells expressing either WT PR or SUMO-deficient mutant K388R PR (phospho-mimic), treated with or without the synthetic progestin, R5020.
Some of these might reflect an inherent greater sensitivity of the microarray technique used for detection of transcript changes, than that of the mass spectrometry method used to probe changes in protein levels.
We have developed an adenoviral microarray technique suitable for miniaturized and parallelized analysis of gene function.
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