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Additionally, the functional assays performed using this practical microarray technique allowed the preliminary identification of several genes involved in the activation of apoptosis, both in tumor and in primary cells.
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The newly available microarray technique allows us to compare the expression profiles of more than 1000 genes that are likely to be associated with oncogenesis in cells at different stages of malignant transformation induced by mycoplasmal infection.
Studies in cancer have validated the effectiveness of the microarray technique, allowing identification of tumor subclasses and marker genes for diagnosis and treatment of the disease (DeRisi et al., 1996; Sorlie et al., 2001).
Microarray techniques allow to detect genome-wide perturbations during various treatments and to measure various responses by multitude of gene probes.
Microarray techniques allow simultaneous measuring of the expression of thousands of genes under different experimental environments and conditions.
Tissue microarray (TMA) technique allows a simultaneous analysis of large amount of tumours on a single microscopic slide.
Another important assumption is forced by the massively parallel experimentation of the microarray technique which allows for assessing expression level of thousands of genes simultaneously.
The ANOVA technique allowed estimating the contribution of several sources of errors to the total variance in the DNA-microarray data of all slides.
The additional sensitivity of this technique allowed for the identification of 1,381 genes (q < 0.05) with overall sex-differential expression, many of which were not identified in our previous microarray study [ 28].
This DNA microarray-based comparative genome sequencing technique allows high resolution detections of sequence polymorphisms [ 26- 28].
In combination with microarray expression analysis, the ChIP-on-chip technique allows identification of direct FOXP3 target genes.
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