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The microarray system was established by determining the method to prepare targets by random-primer labeling with chromosomal DNA and the conditions for hybridization.
In the autoimmune disease setting, the performance of the cellular microarray system was less impressive.
Second, to answer the call for transparency in data documentation [17], [18], we developed a scoring system based on our understanding of what the system was designed to reveal and what the cellular microarray system was measuring.
A two color labeling microarray system was used to compare mRNA expression between primary and DF-1 CEF cells.
A two-colour labelling microarray system was used to compare parental (P) and resistant (R) CALU-3 cell lines.
A two color labeling microarray system was used to compare uninfected- and ILTV infected embryonic lung cells at 1, 3, 5, and 7 dpi.
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A distinct advantage of the Living Microarray system is that expression changes in individual cells can be tracked over time by linking single cells in consecutive frames.
The results of a control experiment confirmed that experimental errors of our microarray system were low (within a 2-fold difference; Supplementary Fig. S1).
Various properties and performance characteristics of our microarray system were measured, which help to understand the nature and limitations of array data.
Currently, several microarray systems are used among which Affymetrix and Illumina platforms are the most popular ones.
A variety of human microarray systems are commercially available and represent well over 30,000 gene and EST sequences.
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