We previously developed a three-dimensional microarray system, the Bio-Strand, which exhibits advantages in automated DNA analysis in combination with our Magtration Technology.
The advantage of the adenoviral microarray system presented in this study is an application of an appropriate blocking solution after printing the adenovirus.
We chose to compare this microarray system against the Taqman qPCR system in particular, since at the time this work was performed this was the most commonly utilized miRNA qPCR system.
The expression of 22,328 genes was detected in at least one condition by this microarray system (Additional file 1: Table S1).
In this microarray system, each gene is represented as 16 distinct pairs of 25-mer oligonucleotide probes.
This microarray system contains total of 14,053 unique cDNAs with 7,937 unique cDNA clones from the neuroendocrine and reproductive systems as well as 9,833 unique cDNA clones from the metabolic and somatic systems [ 24].
Based on a comparison of experimental and theoretical hybridizations, the false-positive and false-negative rates of this microarray system have been calculated as 0.91% and 0.81%, respectively (Sanguin et al. 2006b).
The results indicated that, in this microarray system, as long as the analysis is based on the intensity change by single nucleotide mismatch, the longer probes (>23-mer) are not suitable for accurate measurement of cDNA/cRNA amount due to their low specificity.
This modified microarray system allows direct detection of single nucleotide polymorphisms (SNPs) in human genomic DNA samples without the need for template amplification [ 33].
By investigating more epitopes and shifting the targets from well-established Ag-specific T cell lines to highly heterogeneous clinical samples, our aim was to adapt, simplify and standardize this peptide:MHC cellular microarray system for translational studies.
