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For foreign antigens, which normally trigger robust adaptive responses, the cellular microarray system performed quite well.
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"The system performed flawlessly".
Expression study was performed in a microarray system as described in the Methods.
Microarray hybridization was performed using an Agilent's miRNA microarray system (V12.0), covering 627 mouse miRNAs and 39 mouse viral miRNAs from miRBase database v 12 (Agilent Technologies, Foster City, CA) [ 34].
Global miRNA expression profiling was performed in 17 GISTs using the Human Agilent's miRNA microarray system (Agilent, Santa Clara, CA, USA) with probes matching 903 human miRNAs (miRBase release 14).
We chose to compare this microarray system against the Taqman qPCR system in particular, since at the time this work was performed this was the most commonly utilized miRNA qPCR system.
The labeling and hybridization were performed at Shanghai Biochip Company according to the protocols in the Agilent miRNA microarray system.
Labeling and hybridization were performed at the Shanghai Biochip Company, according to the protocols in the Agilent miRNA microarray system.
We performed microRNA expression profiling starting from 100 ng of total RNA and following the microRNA Microarray System protocol v.1.5 (Agilent Technologies).
miRNA expression profiling was performed on 48 samples on Agilent Human miRNA Microarray Release 14.0, 8x15K slides using Agilent's miRNA Microarray System protocol together with miRNA Spike-In Kit and miRNA Complete Labeling and Hyb Kit.
Genome-wide expression analyses were performed with RNA samples from four fibroblast cell cultures of each twin using the GeneAtlas Personal Microarray System (Affymetrix, Santa Clara, CA, USA).
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