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The oligonucleotides were spotted onto slides of the ArrayTube® microarray system and examined with a new silver-labeling detection technique.
A comparison of the lowest detectable concentration of IgE measured by the PC microarray system and a commercially available clinical analyzer demonstrated that the PCEF microarray system provides higher sensitivity.
The principals of the microarray system and interpretation software have been described (7 ).
These data provide further evidence of solution phase hybridization behavior in the Codelink microarray system and demonstrate that short hybridization times could result in decreased specificity.
A cursory overview of results from the NGL-based microarray system and those from the CFG indicates that with robust carbohydrate-binding systems, there is good agreement.
Note that the expression levels are absolute expression levels that come from a single-channel microarray system and not relative expression levels, where a reference condition is employed.
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A number of altered genes were represented on both the 15 K and 6 K microarray systems and their consistent induction or repression on both arrays gives confidence in the reproducibility of the two systems.
Perhaps surprisingly, correlations achieved between these microarray systems and the independent qRT-PCR technique were higher than the inter-platform comparisons using sequence or Ensembl Gene matching techniques, and even higher than between distinct normalisations on the same platform.
Both studies follow a similar protocol; the study published in the previous issue of Arthritis Research and Therapy used an Affymetrix microarray system [ 1], and an Australian study published online in Annals of the Rheumatic Diseases used an Illumina Chip [ 2].
120 ng of total RNA was labelled and hybridized using the commercial miRNA Microarray System with miRNA Complete and Hyb Kit (Agilent Technologies, Spain) by following manufacturer's instructions.
Evaluation of the immobilization techniques was carried out using both a real-time surface plasmon resonance biosensor system and a microarray system using fluorescent detection of Cy3-labeled target protein.
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