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However, commonly within each individual microarray study, only a small sample size is available, thus the normality assumption may not hold well.
Although this microarray study only assessed a limited amount (1124 sequences) of E. albidus sequences we were able to retrieve 10 orthologous transcripts that show highly comparable significant gene regulation upon Zn exposure.
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Our results raise the question of why c-Myb and other transcription factors are associated with so many promoters, especially since our own microarray studies only identified about 250 genes whose expression levels changed when c-Myb or v-Myb were over-expressed in MCF-7 cells [ 18, 19].
In addition, since our study is based on a population of cells, inherent to all microarrays studies, only measurements of the average cellular behavior are possible.
This value could appear to be rather low, but it has to be compared with other heterologous (Rhesus monkey with human microarrays) or homologous (human with human microarray) studies where only 30 35% and 30 40% of genes were detected ([18]; JDV, personal communication).
However, our microarray studies identified only the 16 functional proteins.
It is common to see microarray studies with only a single time point represented.
A direct comparison of our results with all three microarray studies exhibits only poor overlaps.
Microarray studies can only indicate associations between transcript levels and the phenotype of interest.
Embryo samples were from different pregnancies than those employed for the microarray studies, and only embryos were used that appeared morphologically normal.
Thus, Affymetrix microarray studies using only five mice can prove useful for the identification of gene expression alterations in scrapie-infected mice.
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