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As part of a larger effort to develop lab-on-a-chip methods for detecting radiation exposure events using blood samples, we designed a dose course microarray study in order to determine coding and non-coding RNA transcripts undergoing differential expression immediately following radiation exposure.
The supplemental reference genes were selected based on the data of a previous microarray study in infant rats suffering from PM [20], [38].
This hypothesis is further supported by the observation that our gene expression data were able to identify extensive genetic changes relative to an earlier microarray study in which cell filamentation was induced by an external chemical agent[40].
Finally, we compared our data to a recent microarray study in which a Drosophila line with a mutation in the technical knockout (tko) gene that encodes the mitoribosomal protein S12 was analyzed using Affymetrics platform [38].
A recent DNA microarray study in Cyanothece 51142 showed that diurnal changes in cellular activities such as photosynthesis, respiration, and nitrogen fixation are vastly anticipated at the transcriptional level [5].
To our knowledge, the only description of IL-8 being overexpressed in perihematomal brain areas after ICH is the preceding microarray study in humans [8], although the comparison was done with control subjects.
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Microarray studies were performed in the Finnish DNA Microarray Centre at Turku Centre for Biotechnology.
Experimental design and the selection of animals and samples for microarray studies in farm animals present novel challenges, which are often overlooked.
Fig. 1 MicroRNA that exhibit altered pulmonary expression identified by microarray studies in experimental bronchopulmonary dysplasia.
Finally, we compared our findings with results from other microarray studies in resynthesized allopolyploids.
This criticism is derived from earlier microarray studies in which there was considerable variation between arrays as the result of the array fabrication processes.
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