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This high level of correlation between protein and mRNA behavior was observed in spite of the fact that microarray studies used for comparison were done using yeast from a different strain background (BY4741) than ours (BJ5465), and using different rapamycin treatment conditions (100 nM rapamycin either for 30 minutes [7] or over a time course up to 120 minutes [6]).
This is in contrast to CGH microarray studies used to analyse aberrations in tumour or cancer cells.
Prior c-Myc expression and amplification microarray studies used tumour specimens which contain normal stromal components, potentially underestimating amplification and expression levels of the invasive tumour components (Pollack et al, 2002).
Because all three microarray studies used similar Affymetrix U133A platforms, all probe set measurements were generated using the RMA algorithm in Bioconductor R and related by Affymetrix probe identifiers.
Since these microarray studies used Affymetrix U133A platforms, probe set measurements were generated using the RMA algorithm in Bioconductor R and the resulting data were mapped to our significant gene set by Affymetrix probe identifiers, to which the PAM classifier was applied.
In a review of microarray studies used to explore cancer outcomes, Dupuy and Simon identified the failure to adequately control for false positives as a result of multiple testing as the "most common and serious" flaw they observed in procedures for gene selection [ 5].
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Thus, Affymetrix microarray studies using only five mice can prove useful for the identification of gene expression alterations in scrapie-infected mice.
Knowing that no microarray studies using specific An.
To analyze molecular differences associated with TMC generation, we performed microarray studies using mRNA from pre- and post-senescence MSC, and from TMC.
The present results with marrow ablation-induced intramembranous bone regeneration share similarities with two other microarray studies using rat closed fracture models, which also identified genes for Wnt5a, Frizzled 2, and β-catenin[1], [43].
This is consistent with all previous microarray studies using both long and short MM oligonucleotide probes [ 11, 23].
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