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These microarray studies involved more than 29,000 human genes arrayed on glass slides which contained a total of 43,000 targets [5].
For instance, the early microarray studies involved often only very few replicate samples and they determined differential expression using simplistic statistics, such as fold-change, while it soon became evident that it is essential to consider also the variability over replicate samples.
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In malaria, apoptosis is a process highly represented in the annotation of gene expression profile of acute infection as revealed by several microarray studies involving both human and mouse models [43], [44].
To the end of 2006, there are over 1100 published reports on microarray studies involving the brain and brain disorders, with nearly 100 SAGE studies of the same.
Early microarray studies involving breast cancer cell lines identified a large cluster of coordinately expressed genes associated with cell proliferation rates [ 35].
The relative lack of shared genes with human common host response [ 51] compares favourably with more recent microarray studies involving in vivo immune stimulation of gilthead seabream (Sparus aurata) skeletal muscle cells, human alveolar macrophages [ 20] and leukocytes [ 82].
These human studies involved microarray expression analysis of developing brain, adult brain, and neural progenitors, in that order.
However, few of these studies involved microarray analyses, general expression patterns in cancer cell lines or clinical relativity at the same time.
These were selected from 117 gene probes demonstrating outcome predictive strength in microarray-based studies involving children with septic shock [ 18, 19].
Results of microarray gene expression studies involving fathead minnow, typically used for aquatic ecological toxicology studies, can now be used to generate hypotheses regarding consequences of contaminants and other stressors on humans.
In addition, we highlighted various sources of experimental variations in studies involving microarray gene expression, real-time polymerase chain reaction, proteomics, and other bioinformatics technologies.
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