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Except for complex light harvesting, proteins, microarray studies did not identify significant expression changes in genes of the other proteins, highlighting the importance of proteomic studies.
The adoption of real-time qRT-PCR methodology is somewhat reminiscent of the introduction of cDNA expression microarrays in that initial microarray studies did not identify differentially expressed genes by a statistical method, but by an arbitrary cut-off value of fold-change [ 12].
The array results indicated that Myd88 (a TLR/IL1R adaptor molecule) and Traf6 (TNF receptor superfamily and TLR/IL1R family signaling mediator) were decreased by As exposure as low as 10 ppb, but the microarray studies did not indicate a change in IκBα.
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The other two microarray studies done with CEU LCLs contained fewer IRF5 probes.
There remains considerable debate in the literature as to why comparative microarray studies do not completely coincide with each other.
Likewise, lack of expression in microarray studies does not necessarily exclude a gene from being involved in models other than the one in which the studies were undertaken.
There remains considerable debate in the literature as to why comparative microarray studies do not completely coincide with each other [ 10].
In addition, many microarray studies do not match the MAQC platform quality, experimentation expertise and relative high signal-to-noise ratios of the samples compared, and would thus generate data of yet poorer reliability.
As with other microarray studies done on older mammalian muscle [ 13- 17], we have also found a disproportionate number of metabolic genes, such as those involved in lipid and carbohydrate metabolism, decrease in transcription levels with age.
In agreement with previous microarray studies done on skeletal muscle of mice, monkeys and humans, we also found that genes associated with metabolic functions, particularly energy pathways, were higher in young control animals (Table 1).
As noted previously, most microarray studies do not involve uniform temporal sampling of the state of a system where inferred relationships in the gene expression state space of genes can more easily be detected.
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