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We then noted two features of the sequence data that may explain in part the large signals that the outlier microarray spots exhibited when hybridized to the oligo dT) probe.
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All protein spots exhibited molecular weights (MWs) of 10 175 kDa.
The imaged spots exhibit wavelength-dependent absorption and scattering signals.
The protein spots exhibiting ±2-fold differences in relative volume were considered differential spots.
The laser damage spot exhibited a ring shape.
Accordingly, this spot exhibited lower SA in ε4-positive patients.
In general, genes exhibiting late Ct values corresponded to low intensity microarray spots: 75.6% of genes with Ct values above the median exhibited below-median spot intensity, and thus likely represent genes with low expression levels.
The scatterplot exhibits only a weak correlation between these two hybridization results, for the vast majority of microarray spots.
The segmentation of cDNA microarray spots is essential in analyzing the intensities of microarray images for biological and medical investigation.
Some of these data sets were obtained using cDNA microarrays, which may have spotted chromosomal neighbors onto neighboring microarray spots.
Figure 1a displayed the SEM image of the 2D VO2 microarrays (designated as VO2-N microarrays), in which the microarrays exhibited uniform structure with a size over several millimeters.
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