Clinical and histopathological data of the subset of 33 patients with cc-RCC considered for immunhistochemical (n=16 patients) or immunofluorescence (n=17 patients) analysis of paraffin embedded tissue microarray specimens are shown in Table 2.
Microarray analyses on 58 frozen specimens were performed using Human 1A Microarray (V2) G4110B (Agilent technologies).
Biopsy specimens were microarrayed.
cDNA microarray expression data from normal liver specimens were previously published.[12] A tissue arrayer (Beecher Instruments, Sun Prarie, WI) was used to construct a primary CRC tissue microarray as described,[48] comprising of 154 primary colorectal tumors each represented by two 6-mm cores.
Microarray expression profiles of 476 neuroblastoma specimens were generated and genes differentially expressed between favorable and unfavorable neuroblastoma were identified.
The specimens were arranged into microarray using the immunohistochemistry method to investigate the expression of integrin αvβ6 and transcriptional factor Ets-1 in these tissues.
In brief, tissue specimens were prepared for microarray format by selecting representative breast tumor areas from 651 formalin-fixed, paraffin-embedded primary tumor blocks using hematoxylin and eosin stained whole-section slides.
In addition to the ependymoma variants, 50 other pediatric central nervous system (CNS) tumor specimens were analyzed by microarray including 18 glioblastomas (GBM), 10 pilocytic astrocytomas (PA), nine atypical teratoid/rhabdoid tumors (ATRT), nine classical medulloblastomas (MED) and four large-cell medulloblastomas (LCM).
Formalin-fixed, paraffin-embedded tumor specimens were used for tissue microarray construction.
A total of 115 endometrial cancer specimens and five normal endometrial specimens were included on the microarray slides.
