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Samples were then evenly dispersed onto microarray slides with cover-slips by capillary action.
The DNA was UV cross-linked to the microarray slides with a Stratalinker at 300 mJ (Stratagene, La Jolla, CA).
Hybridization was performed on mouse microarray slides with 22000 prints (G4112A, Agilent).
Microarray slides with ~46K features were manufactured by the University of Arizona Maize Array Project (http://www.maizearray.org).org
For Arabidopsis genes with no available cDNA sequence, microarray slides with highest transcript levels within the NASCArrays data set [ 35] have been detected using the Spot History tool.
The technology is based on attaching DNA fragments, or probes, to microarray slides, with each probe having exact nucleotide sequence complementarity to a specific transcript.
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Figure 2 shows the results of multivariate data analysis of a hyperspectral image from a microarray slide with contaminating fluorescence.
Fluorescently labeled targets of control (sham) as well as treated (PMCAO) samples were hybridized to the same microarray slide with 60-mer probes (Fig. 1C).
This is because a rather big loop would be needed for our study of 10 treatment groups and the design of the microarray slide with 4 arrays per 1 slide would result in a large loss of data if a slide were to fail.
For quality control, the microarray slides printed with rSEPs were incubated with mouse anti-His tag IgG-Cy5 (SBA, Birmingham, AL) and scanned for their FI values.
Data were taken from microarray slides hybridised with labelled cDNA from bovine PBLs stimulated with ConA.
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