Within the last decade, the development of antigen microarray slides has enabled the simultaneous measurement of serum reactivity to hundreds of peptides in a single biological sample.
Soft-tissue sarcoma microarray slides had a total of 151 specimens (Table 1).
The sarcoma tissues used here were classified according to clinicopathological data in Table 1 and 2. Osteosarcoma tissue microarray slides had a total of 113 specimens.
Rhabdomyosarcoma tissue microarray slides had a total of 64 specimens in which 32 of them were ARMS and another 32 specimens were ERMS.
Comparative Genomic Hybridization (CGH) using microarray slides has emerged as a powerful tool to quantify genomic deletions and copy number variants [ 20].
The data obtained from NASCArrays, from 72 experiments comprising of 956 Affymetrix ATH1 microarray slides, had already been normalized with the Affymetrix MAS 5.0 algorithm to the common mean value of 100.
These labeled products from both groups are mixed well in equal amount and blotted on differential methylation hybridization (DMH) microarray slide having printed human CGI library probes (Schumacher et al., 2006).
Each microarray slide has 21 synthesized arrays, each representing all of the 1050 miRNAs plus housekeeper genes, in separate wells in a design that mimics standard SBS 96-well foot print using ArraySlide 24-4 Frame gasket (The Gel Company, San Francisco, CA, USA), permitting 24 samples to be tested per slide.
The probe was applied to a microarray slide that had been pre-hybridised for 2 hours with hybridisation buffer.
This purification process has been adapted to the protein microarray format, but the quality of in situ His-tagged protein purification on slides has not been systematically evaluated.
