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Agilent (Palo Alto, CA, USA) G2519F porcine gene expression microarray slides containing more than 40,000 probes were used for this experiment.
Custom Agilent microarray slides containing 10 probes per array complementary to 40 nucleotide selected region of each SOMAmer with a 20x dT linker were placed onto the gasket slides according to the manufacturer's protocol.
Labeled aRNA was dissolved in 90 µl of hybridization buffer, denaturated at 90°C for 3 min and applied directly to human OpArrays DNA microarray slides containing 35130 spotted oligonucleotide sequences representing 29166 different human genes (Operon Biotechnologies, Inc).
Total RNA was isolated from log phase cultures of mycobacteria containing either hcl1pVV16 or pVV16, labelled with Cy3/Cy5 dyes and hybridized to microarray slides containing 4750 oligonucleotides (70-mer), each representing a unique M. tuberculosis H37Rv gene.
High density normal tissue microarray slides containing 45 normal human tissues in duplicate (AccuMax Array # A103VI-Normal tissues) were purchased from ISU-ABXIS (Accurate Chemical Corp ,Westbury, NY, USA).
Human prostate tissue microarray slides containing individual tumour cores (1.5 mm diameter, 5 μm) were purchased.
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The microarray slides contain 15,208 probes for S.cerevisiae (BY4742 strain).
The microarray slides contained 8 identical arrays per slide, which could each be individually hybridized [ 14].
Glass microarray slides contain thousands of nucleic acid features that can be interrogated in parallel.
Microarray slides contained approximately 33.000 oligonucleotides corresponding to the whole mouse genome (AECOM, USA, http://microarray1k.aecom.yu.edu/).aecom.yu.edu/
Labeled cDNA from each of the eight time points was hybridized to a microarray slide containing 8 separate copies of the E. coli genome (Agilent Microarray Design ID 020097) according to instructions provided by the manufacturer.
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