Agilent (Palo Alto, CA, USA) G2519F porcine gene expression microarray slides containing more than 40,000 probes were used for this experiment.
High density normal tissue microarray slides containing 45 normal human tissues in duplicate (AccuMax Array # A103VI-Normal tissues) were purchased from ISU-ABXIS (Accurate Chemical Corp ,Westbury, NY, USA).
Custom Agilent microarray slides containing 10 probes per array complementary to 40 nucleotide selected region of each SOMAmer with a 20x dT linker were placed onto the gasket slides according to the manufacturer's protocol.
Total RNA was isolated from log phase cultures of mycobacteria containing either hcl1pVV16 or pVV16, labelled with Cy3/Cy5 dyes and hybridized to microarray slides containing 4750 oligonucleotides (70-mer), each representing a unique M. tuberculosis H37Rv gene.
Labeled aRNA was dissolved in 90 µl of hybridization buffer, denaturated at 90°C for 3 min and applied directly to human OpArrays DNA microarray slides containing 35130 spotted oligonucleotide sequences representing 29166 different human genes (Operon Biotechnologies, Inc).
The microarray slides contained 8 identical arrays per slide, which could each be individually hybridized [ 14].
Human prostate tissue microarray slides containing individual tumour cores (1.5 mm diameter, 5 μm) were purchased.
An external GEX dataset on custom made cDNA microarray slides containing 16,457 sequence-verified I.M.A.G.E.
Microarray slides contained approximately 33.000 oligonucleotides corresponding to the whole mouse genome (AECOM, USA, http://microarray1k.aecom.yu.edu/).aecom.yu.edu/
