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Custom made microarray slides, consisting of 44,000 60-mer oligonucleotide probes covering the entire S. venezuelae genome, were designed and produced by Oxford Gene Technologies (Oxford, UK).
In brief, two tissue microarray slides consisting of human breast cancer (140 and 70) cores and adjacent benign breast tissue (10 and 24) cores were purchased from the Biomax US (Rockville, MD, USA).
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The microarray slides consisted of 5,760 amplified cDNA fragments from soybean libraries prepared from RNA of developing seeds [ 86].
Each microarray slide consisted of approximately 13,000 elements including target genes and quality controls.
For immunohistochemical analysis, 4 μm tissue microarray (TMA) slides consisting of triplicate punches of 103 cervical cancer patients were deparaffinised and rehydrated.
It is a slide consisting of more slides.
Prostate tissue microarray slides were prepared at the New York University Medical Center and consisted of a total of 132 cases.
We designed and ordered custom DNA microarrays through Agilent, where each slide consisted of eight identical subarrays of 15 000 individual features.
Microarray slides were gridded with the 'ICR-geneset' that consisted of 5603 I.M.A.G.E cDNA clones (including 169 duplicates) acquired from the UK Human Genome Mapping Project Resource Centre and Research Genetics (http://www.resgen.com).
The microarray experiment therefore consisted of a total of 48 microarray slides.
Individual microarray features were spotted in duplicate, hence each slide consists of 11,710 spots (technical replicate) representing 5,855 unique features.
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