Microarray slides comprising ∼15,000 expressed sequence tags from the National Institute of Ageing 15 K mouse clone library (Australian Genome Research Facility, Melbourne, Australia) were hybridized with Cy3- and Cy5-labeled cDNA generated from double-amplified RNA.
Immunohistochemical staining of HOXB13 was performed on tissue microarray slides comprising a total of 912 tumors from breast cancer patients.
Therefore, the microarray slides comprised 45,220 features representing 1,417 eArray internal controls and 600 internal controls representing 60 randomly chosen ORFs (10 replicates).
Therefore, the microarray slides comprised 45,220 features including 1417 Agilent internal controls and 800 internal controls that represented 80 randomly chosen A. nidulans ORFs (each 10-times replicated).
The data obtained from NASCArrays, from 72 experiments comprising of 956 Affymetrix ATH1 microarray slides, had already been normalized with the Affymetrix MAS 5.0 algorithm to the common mean value of 100.
A total of 48 cDNA microarray slides were used for this study, and each slide was comprised of 6119 unique gene sequences and 2329 EST sequences printed in duplicate on each slide.
Spotted microarray slides were obtained from the Pathogen Functional Genomics Resource Center at JCVI.
We thank the Pathogen Functional Genomics Resource Center at JCVI for providing S. sanguinis spotted microarray slides.
All microarray slides were incubated using an 8 well gasket slide and incubation cassette system (Agilent Technologies) and were protected from light throughout the procedure.
Peptide microarray slides usually contain positive control spots to gauge for antibody binding.
