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The 4 × 44 k rabbit whole genome microarray slides, associated reagents and software were purchased from Agilent Technologies (Agilent Technologies, Inc. Santa Clara, CA) and used as per the manufacturers' instructions.
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Spotted microarray slides were obtained from the Pathogen Functional Genomics Resource Center at JCVI.
We thank the Pathogen Functional Genomics Resource Center at JCVI for providing S. sanguinis spotted microarray slides.
All microarray slides were incubated using an 8 well gasket slide and incubation cassette system (Agilent Technologies) and were protected from light throughout the procedure.
Peptide microarray slides usually contain positive control spots to gauge for antibody binding.
The microarray slides were custom-designed and contained 101,720, 60 mer probes.
RNA from E14.5 embryonic hearts was isolated and then blotted with microarray slides.
Transcriptomic raw data were obtained by scanning of the microarray slides using Illumina GenomeStudio V2010.3.
Hybridization and washing of microarray slides (GPL7252, 43494 probes [Edgar et al. [2002]) were performed following manufacturer's protocols.
We thank PFGRC for providing microarray slides.
The microarray slides were scanned using ScanArray Express.
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