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Assuming microarray signals relate directly to enzymatic activity, our working hypothesis is that particle-attached organisms, which encounter low-oxygen microenvironments, expressed the genes for dissimilatory nitrate reduction in the water column, while free-living, or both particle-attached and free-living microorganisms, expressed nitrate assimilation genes.
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Cortical signals related to gains and losses.
Our microarray data is reliable, because some expected Notch signaling related genes did appear in the transcriptome, such as up-regulated heyL and down-regulated dla.
By fitting a Gaussian mixture model to AMR-related signal intensities, we provide an indication of the reliability of microarray signals.
We selected genes participating in our network that were significant by microarray analysis in both direct and bystander irradiation, such as cytokine-chemokine signaling related genes including PTGS2, IL6, IL8, CXCL2 and CXCL3.
The microarray signals were analyzed using the Affymetrix RMA algorithm.
Microarray signals were determined using Affymetrix Microarray Suite 5.1.
To compare qPCR-array and microarray assays, the log2 of microarray signals was used.
The microarray signal was extracted using GenePix.
The presence of miRNA was determined based on a corresponding microarray signal of greater than [mean + 2 × standard deviation] of the negative control signals, from which the most and least intense signals were removed.
After fully correcting the subtype from the result of microarray signal intensity, the microarray output method combined with bioinformatics tools, identified and monitored genetic variations of H5N1.
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