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Based on the microarray sequences used to design our PCR primers, the cyp1a1 assay matched equally well against cyp1a3 with BlastX searches, while the cyp1c1 assay matched almost equally against cyp1c2, suggesting that more research are needed into the transcription of the different CYP1 genes and organ-specific function of their encoded proteins in cod.
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These were either sequence pairs that did not show significant intensity differences in the microarray experiments or individual sequences used as internal controls for the QPCR experiments.
Primer sequences used for microarray validation have been previously published [ 64].
Primers were designed from the barley sequences used for microarray probe design (Supplementary Data 2, available online).
This approach is also able to identify chromosomal discontinuities which when coupled to MME analysis can identify new sequences and genes not present in any of the sequences used for microarray design.
IM developed the contig sequences used for the microarray.
Eclat classification of the lichen EST sequences used for the microarray probe design was performed as previously described [ 13].
The Contigs and singleton sequences used on the microarray were used to query GenBank's nonredundant database and the TAIR database with BLASTx.
The genome of origin for the lichen EST sequences used in the microarray probe design was predicted using Eclat [ 26]. 29.6% of the EST sequences used in the microarray probe design are predicted to be of algal origin while the remaining 70.4% are predicted to be fungal sequences.
The process was repeated with different random seeds and the number of required probe sequences did not vary significantly while the sequences used in the microarray design could change.
All EST sequences used to fabricate microarrays have been submitted to dbEST at GenBank [ 46].
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