In contrast, for microarrays, sensitivity is limited by the inherent signal:noise ratio of the read-out technology itself, rather than only biological noise.
Chromosomal microarray offers superior sensitivity for identification of submicroscopic copy number variants (CNV) and it is advocated to be the first tier genetic testing for patients with autism spectrum disorder (ASD).
Thus, the distribution of the RA values from 454-sequencing is considerably wider than that observed for microarray, indicating that sensitivity for detecting expression difference across tissue samples is highest for 454-sequencing.
For the heterogeneous assay of nucleic acid on DNA microarrays, sensitivity is one of the most important factors and special emphasis has been given to recently developed signal amplification strategies aimed at achieving high sensitivity.
DNA microarrays are among the most widely used technical platforms for DNA and RNA studies, and issues related to microarrays sensitivity and specificity are therefore of general importance in life sciences.
RNA-Seq offers several advantages over hybridization-based approaches such as microarrays, including a markedly higher sensitivity for low abundance transcripts, single nucleotide resolution of transcript boundaries, and the means to profile gene expression in strains for which genome sequences and/or gene annotations are not available [ 12, 13].
From these data, 10 pg of synthetically labeled target, equivalent to 30 fM per target, was needed to achieve appropriate specificity and sensitivity for the constructed microarrays.
The intended users of the database include any genomics researchers facing the persistent challenges of sensitivity for biomarker discovery and cross-platform microarray comparisons.
However, microarray technology suffers from well-known limitations including insufficient sensitivity for quantifying lower abundant transcripts, narrow dynamic range and non-specific hybridizations.
Specificity and sensitivity for 25 signal cut-offs from 2 microarray experiments.
