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Tissue microarray sections were dewaxed in xylene and rehydrated, then unmasked in retrieval solution (S2367, DAKO) at 121°C in a pressure cooker for 5 minutes.
Tissue microarray sections were deparaffinised in xylene and rehydrated.
Tissue microarray sections were stained immunohistochemically for CA IX (M75).
Tissue microarray sections were deparaffinized and rehydrated through a graded ethanol series finishing with distilled water.
Briefly, tissue microarray sections were deparaffined in xylene and rehydrated with graded ethanol.
Tissue microarray sections were deparaffinised and rehydrated through a series of graded alcohols.
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WEE1 immunohistochemical distribution on tissue microarray sections was analysed by two of the authors (JR-F and KS) on a multi-headed microscope.
Caveolin-1 and CAV2 immunohistochemical distribution on tissue microarray sections was analysed by three of the authors (ER, RS & AA), separately.
Tissue microarray sections are counterstained with hematoxylin, enabling a nonspecific visualization of microscopic structures, in addition to the binding of the antibody that results in a dark-brown stain 31.
Tissue microarray (TMA) sections were deparaffinised using xylene and dehydrated in alcohol.
Tissue microarray (TMA) sections were immunoassayed for Ki-67 using MIB-1 antibody, DAKO, Carpinteria, CA, USA, as detailed previously (Berney et al, 2009).
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