Sentence examples for microarray screening was from inspiring English sources
In order to identify human proteins interacting with the Neisserial adhesin NadA12, a large-scale protein microarray screening was performed spotting 2354 human recombinant proteins from the GNF library.
The protein microarray screening was performed testing 159 purified recombinant S. aureus proteins spotted onto nitrocellulose coated glass slides against 75 human recombinant proteins that include human complement factors and extracellular matrix proteins (Fig. 2a and Supplementary Table S1).
Whole-genome microarray screening was performed for each sample.
The initial interaction data derived from microarray screening was further analyzed by fluorescence polarization.
However, Sult2a2, the gene increased most strongly in microarray screening, was shown by RT-PCR to be elevated by more than 1000-fold in KO mice.
Two candidate genes (CENP-F and CDC6) detected on the cDNA microarray screening were selected for the validation of the microarray results.
The initial microarray screen was uninformative with respect to interferon signalling, but this was of interest because soluble gD has been reported to induce IFN [29], while, in contrast, ISG induction is reported to require virion entry but not de novo protein synthesis [30].
A miRNA expression microarray screen was used to analyse SW480 and HCT116 colorectal and MDA-MB231 breast cancer cell clones with stable short hairpin RNA-mediated knockdown of ZEB1 (shZEB clones) in comparison with control knockdown (shCtl) clones.
To further characterize the agp6 agp11 mutant line a differential microarray screen was carried out to identify genes with modified expression in the 8 h-grown pollen tubes of agp6 agp11 compared to wild type pollen tubes.
To gain an insight into which of these genes was more likely to be involved in modulating Aβ toxicity in the fly, an Affymetrix cDNA microarray screen was undertaken to search for genes that were up- or down-regulated in flies expressing Arctic Aβ42 as compared to flies expressing the non-toxic Aβ40 peptide [44].
The microarray screens were conducted at the DNA Microarray facility of UCLA, Los Angeles.
