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It is critical that the findings of microarray screening methodologies are validated to produce scientifically robust results, using the most sensitive and reproducible method of gene expression quantitation, reverse transcription quantitative PCR (RT-qPCR) [ 16].
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A greater understanding of the biological mechanisms within organisms becomes possible with the availability of complete genome data, in combination with high-throughput screening methodologies such as microarrays.
The availability of genome sequences of a variety of species, the development of high throughput screening methodologies such as DNA microarrays, and the refinement of computational algorithms for searching large databases have all stimulated more and more genome-wide investigations.
The paper focuses on virtual screening methodologies.
Two different microarray screening setups were designed for the two pathogens, trying to answer different biological questions.
Methods for assessing the function of new candidate genes identified in microarray screening experiments are also discussed.
Microarray screening assays revealed 18 hybridization maps.
The integrated panel of microbial screens and standard screening methodologies were adopted as previously described [ 14].
Only 5 μg of protein were necessary to perform the whole procedure, since we took advantage of methodologies that require minimal amounts of protein: i) in vitro protein expression and purification, ii) antibody phage display and iii) scFv antibody microarray screening.
RP participated in biochemical studies, microarray screening and RT-qPCR.
Small-molecule microarray screening for TFIID-specific modulators.
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